publicações selecionadas
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artigo académico
- Candida albicans chitinase 3 with potential as a vaccine antigen: production, purification, and characterisation. Antibiotics. 2024
- Endo-1,4-ß-xylanase-containing glycoside hydrolase families: characteristics, singularities and similarities. Biotechnology Advances. 2023
- Psychrophilic enzymes: strategies for cold-adaptation. Essays in Biochemistry. 2023
- Development of new chimeric proteins for tissue engineering. International Review of Neurobiology. 2013
- Cold-Adapted enzymes: fundamentals and biotechnological aspects. Applied Biotechnology. 2009
- Cold-adapted enzymes from marine Antarctic microorganisms. Marine Biotechnology. 2006
- A nondetergent sulfobetaine prevents protein aggregation in microcalorimetric studies. Analytical Biochemistry. 2006
- Oligosaccharide binding in family 8 glycosidases: crystal structures of active-site mutants of the ß-1,4-xylanase pXyl from Pseudoaltermonas haloplanktis TAH3a in complex with substrate and product.. Biochemistry. 2006
- Psychrophilic microorganisms: challenges for life. Embo Reports. 2006
- Use of glycoside hydrolase family 8 xylanases in baking. Journal of Cereal Science. 2006
- Study of the active site residues of a glycoside hydrolase family 8 xylanase. Journal of Molecular Biology. 2005
- Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiology Reviews. 2005
- A perspective on cold enzymes: current knowledge and frequently asked questions.. Cellular and Molecular Biology. 2004
- Some like it cold: biocatalysis at low temperatures. FEMS Microbiology Reviews. 2004
- Extreme catalysts from low-temperature environments. Journal of Bioscience and Bioengineering. 2004
- Activity, stability and flexibility in glycosidases adapted to extreme thermal environments. Journal of Molecular Biology. 2003
- The structure of a cold-adapted family 8 xylanase at 1.3 Å resolution: structural adaptations to cold and investigation of the active site.. Journal of Biological Chemistry. 2002
- Crystallization and preliminary X-ray analysis of a xylanase from the psychrophile Pseudoalteromonas haloplanktis. Acta Crystallographica Section D Biological Crystallography. 2002
- Molecular basis of cold adaptation. Philosophical Transactions of the Royal SocIETy B: Biological Sciences. 2002
- A novel family 8 xylanase, functional and physicochemical characterization. Journal of Biological Chemistry. 2002
- Did psychrophilic enzymes really win the challenge?. Extremophiles. 2001
- Cold-adapted enzymes: from fundamentals to biotechnology. Trends in Biotechnology. 2000
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artigo de conferência
- Optimising a cell factory system for the bioproduction of silk-elastin-like polymers. 2013
- A novel family 8 psychrophilic xylanase: fundamentals and applications 2005
- Adaptation strategies and use of cold-adapted enzymes in biotechnological processes 2005
- Fundamental and biotechnological aspects of cold-adapted enzymes. 2005
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artigo de revista
- Design of a lipid nano-delivery system containing recombinant Candida albicans chitinase 3 as a potential vaccine against fungal infections. Biomedicine & Pharmacotherapy. 2023
- Development and Characterization of Monoolein-Based Liposomes of Carvacrol, Cinnamaldehyde, Citral or Thymol with anti-Candida Activities.. Antimicrobial Agents and Chemotherapy. 2021
- Psychrophilic lifestyles: mechanisms of adaptation and biotechnological tools. Applied Microbiology and Biotechnology. 2019
- Microbial ecology of the cryosphere (glacial and permafrost habitats): current knowledge. Applied Microbiology and Biotechnology. 2019
- Conditions promoting effective very high gravity sugarcane juice fermentation. Biotechnology for Biofuels. 2018
- Quantitative assessment of DNA damage in the industrial ethanol production strain Saccharomyces cerevisiae PE-2. FEMS Yeast Research. 2018
- Deciphering the factors defining the pH-dependence of a commercial glycoside hydrolase family 8 enzyme. Enzyme and Microbial Technology. 2017
- Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia. 2017
- Antibiotic free selection for the high level biosynthesis of a silk-elastin-like protein. Acta Cytologica. 2016
- Activity-stability relationships revisited in blue oxidases catalyzing electron transfer at extreme temperatures. Extremophiles. 2016
- Exploring the Properties of Genetically Engineered Silk-Elastin-Like Protein Films. Acta Biomaterialia. 2015
- Development of Elastin-Like Recombinamer Films with Antimicrobial Activity. Biomacromolecules. 2015
- DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: A novel adjuvant and delivery system. European Journal of Pharmaceutics and Biopharmaceutics. 2015
- High Level Biosynthesis of a Silk-Elastin-like Protein in E. coli. Biomacromolecules. 2014
- Batch production of a silk-elastin-like protein in E. coli BL21(DE3) : key parameters for optimisation. Microbial Cell Factories. 2013
- High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures. Amb Express. 2013
- Backbone and side chain 1H, 15N and 13C assignments for a thiol-disulphide oxidoreductase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125. Biomolecular NMR Assignments. 2010
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capítulo de livro
- Inverse PCR for site-directed mutagenesis 2023
- Biotechnological aspects of cold-active enzymes 2017
- Enzyme catalysis in psychrophiles 2017
- Inverse PCR for point mutation introduction. Methods in Molecular Biology. 2017
- Psychrophiles: from biodiversity to biotechnology 2017
- Cold-adapted enzymes: fundamentals and biotechnological aspects 2009
- Fundamentals of cold-adapted enzymes 2008
- Cold-adapted enzymes 2007
- Microcalorimetry as applied to psychrophilic enzymes 2005
- Life in the cold: psychrophilic enzymes 2002
- Cold-adapted enzymes 2001
- Cold-adapted enzymes: an unachieved symphony 2001
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documento
- Unlocking the Biotechnological Potential of Marine Microorganisms: The River2Ocean Project´s Contribution to the Blue Economy 2023
- Unlocking the biotechnological potential of marine microorganisms: the River2Ocean project's contribution to the blue bioeconomy 2023
- A novel approach for site-directed mutagenesis: process optimisation. 2023
- The River2Ocean project: isolation, genotyping and phenotyping of marine microorganisms as key drivers for sustainable innovation. 2023
- Extraction of xylan from Palmaria palmata. 2023
- Marine Microbial Resources: Paving the Way for a Blue Bioeconomy 2023
- Marine microbial resources: paving the way for a blue bioeconomy. 2023
- Establishment of a biobank of marine microorganisms with biotechnological potential 2023
- Establishment of a biobank of marine microorganisms with biotechnological potential. 2023
- A novel method for single and multi-site-directed mutagenesis. 2022
- Candida albicans chitinase Cht3: a novel vaccine antigen against fungal systemic infections. 2022
- Antifungal activities of carvacrol, cinnamaldehyde, citral and thymol liposomes against Candida. 2019
- Characterisation and liposomal encapsulation of recombinant Candida albicans Cht3 - a novel subunit antigen against fungal infections. 2018
- Candida albicans Cht3 as a novel recombinant subunit antigen against fungal infections: characterisation and liposomal encapsulation. 2018
- Quantitative assessment of DNA damage in the industrial ethanol production strain Saccharomyces cerevisiae PE-2. 2018
- Deciphering the role of exposed surface residues in protein stability. 2017
- Investigating the role of exposed surface residues in protein stability. 2017
- Enhancement of the biotechnological value of a commercial cold-adapted xylanase. 2017
- Exploring the Antarctic (Explorando a Antártida) 2017
- Life at the extremes 2016
- Heterologous production and purification of Candida albicans Cht3 and Sap2: novel recombinant subunit antigens. 2016
- High-level biosynthesis of a silk-elastin-like protein in E. coli: fed batch production and antibiotic free selection. 2016
- Protein engineering for low pH stability: the role of exposed surface residues. 2016
- Silk-based biomaterials with improved cell adhesion properties 2016
- Engineering a commercial cold-adapted xylanase for improved acid pH stability. 2016
- Expression of a cold adapted xylanase in Saccharomyces cerevisiae for xylan metabolisation. 2016
- Genetically engineered silk-based composite biomaterials functionalized with fibronectin type-II that promote cell adhesion 2016
- Production and purification of Candida albicans Cht3 and Sap2 in E. coli: novel recombinant subunit antigens. 2016
- Investigating the Role of Surface Exposed Residues in the Acidic pH Instability of a Commercial Xylanase. 2015
- Reducing Acetate Accumulation and Plasmid Instability for Enhanced Recombinant Protein Production in E. coli 2015
- Silk-based polymers functionalized with fibronectin type-II for improved cell adhesion. 2015
- Towards functional screening for xylo-oligomer transporters. 2015
- A vida no extremo 2015
- Developing a recombinant Saccharomyces cerevisiae strain for growth on xylan. 2015
- Enhancing stability and activity of a family 8 xylanase at acidic pH. 2015
- Nanocellulose composites using a recombinant protein based polymer as matrix. 2015
- Engineering a commercial cold-adapted xylanase for enhanced biotechnological value. 2014
- High-level biosynthesis of a silk-elastin-like protein in E. coli: reducing acetate accumulation and plasmid instability. 2014
- Fabrication of SELP/Ag nanocomposite materials with antimicrobial properties by electrospinning and solvent casting. 2014
- High level biosynthesis of a silk-elastin-like protein in E. coli. 2014
- Optimisation of the fed-batch production of a silk-elastin-like protein: overcoming acetate accumulation and plasmid instability. 2013
- Development of new chimeric proteins for tissue engineering. 2013
- Life at the Extremes (A vida no extremo) 2013
- Novel nanocellulose reinforced recombinant protein based polymer composites. 2013
- Synthesis and processing of recombinant silk and elastin copolymers. 2013
- Key stress factors and parameters for production optimisation of silk-elastin-like proteins in E. coli BL21(DE3). 2013
- Key stress factors and parameters for batch production optimisation of silk-elastin-like proteins in E. coli. 2011
- NMR Solution structure of a cold-adapted thiol-disulfide oxidoreductase. 2011
- NMR solution structure of a cold-adapted thiol-disulfide oxidoreductase. 2010
- NMR solution structure determination of a psychrophilic thiol-disulfide oxidoreductase. 2008
- Isolation of novel hydrolytic genes from an Antarctic metagenomic library. 2008
- A novel family 8 psychrophilic xylanase: fundamentals and applications. 2006
- Cold-adapted xylanases: from Antarctica to the table. 2006
- Activity, stability relationships in extremophilic enzymes. 2005
- A psychrophilic family 8 xylanase acts as a novel efficient improver of bakery products. 2004
- A novel family 8 psychrophilic xylanase: fundamentals and applications. 2004
- Activity, stability relationships in extremophilic enzymes. 2004
- Structural adaptations of psychrophilic enzymes. 2004
- A novel family 8 xylanase: characteristics and evolutionary aspects. 2001
- Study of thermostabilized mutants of the psychrophilic Pseudoalteromonas haloplanktis xylanase. 2001
- Molecular adaptations to cold: cold adapted bacterial xylanases. 2000
- Lake vostok: molecular adaptations to cold. 1999
- 1H13.pdb: Structure of a cold-adapted family 8 xylanase (wild-type)
- 1H14.pdb: Structure of a cold-adapted family 8 xylanase (mutant D144N)
- 1XW2.pdb: Structure of a cold-adapted family 8 xylanase (mutant E78Q)
- 1XWT.pdb: Structure of a cold-adapted family 8 xylanase (mutant D281N)
- 2A8Z.pdb: Structure of a cold-adapted family 8 xylanase (mutant D144A)
- BMRB Entry 16689: Backbone and side chain 1H, 15N and 13C assignments for a cold-adapted thiol-disulphide oxidoreductase
- Pseudoalteromonas haloplanktis xyl gene for endo-1,4-beta-xylanase, GenBank: AJ427921.1
- Structure of a cold-adapted family 8 xylanase (1H12): WT-xylose (X2)
- Structure of a cold-adapted family 8 xylanase in complex with substrate (2B4F): D144A-X5
- Structure of a cold-adapted family 8 xylanase: E78Q-X3 (1XWQ)
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teses